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(A-D) OMVs from ETBF and NTBF were administered to HEK-293T cells expressing the indicated TLR proteins and an NFκB-responsive SEAP reporter gene. TLR activity was calculated by comparing vesicle and positive control treatments (gray columns) to the corresponding TLR cells treated with PBS (PBS treatment activity is represented by the dashed lines at y = 1). Points on the graph represent biological replicates (n=3) and data is presented as mean ± S.E.M. Statistical significance was found using Dunnett’s multiple comparison test. Stars represent significantly different from PBS control. ** p < 0.01, **** p < 0.0001. LPS = TLR4 agonist, Poly I:C = TLR3 agonist, CL307 = TLR7 agonist.

Journal: bioRxiv

Article Title: Outer membrane vesicles from Bacteroides fragilis contain coding and non-coding small RNA species that modulate inflammatory signaling in intestinal epithelial cells

doi: 10.1101/2025.06.25.661399

Figure Lengend Snippet: (A-D) OMVs from ETBF and NTBF were administered to HEK-293T cells expressing the indicated TLR proteins and an NFκB-responsive SEAP reporter gene. TLR activity was calculated by comparing vesicle and positive control treatments (gray columns) to the corresponding TLR cells treated with PBS (PBS treatment activity is represented by the dashed lines at y = 1). Points on the graph represent biological replicates (n=3) and data is presented as mean ± S.E.M. Statistical significance was found using Dunnett’s multiple comparison test. Stars represent significantly different from PBS control. ** p < 0.01, **** p < 0.0001. LPS = TLR4 agonist, Poly I:C = TLR3 agonist, CL307 = TLR7 agonist.

Article Snippet: HEK-Blue TLR cells were additionally supplemented with 100 μg/mL of Normocin (Invivogen, ant-nr-1), 1X HEK-Blue Selection (Invivogen, hb-sel), 100 μg/mL of Zeocin (Invivogen, ant-zn-05), and 30 μg/mL of blasticidin (Invivogen, ant-bl-05).

Techniques: Expressing, Activity Assay, Positive Control, Comparison, Control